Re: Avoiding gelatine ---agar as an alternative? (fwd)

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From: Breukel, C (HKG) (cor@lumc.nl)
Date: 07/19/02-03:31:24 AM Z


I also use lot of agar every day. As agar-agar (crude form) in
petri-dishes, for growing bacteria etc. And in it's purified form as
agarose, mainly used to separate (based on length) of DNA (and RNA)
fragments. Funny that you can use it as a size, but does one need to
harden it? I assume so. I guess you also have to protect it from the
little critters who like to dine on it.

Best

Cor

"Gordon J. Holtslander" wrote:
>
> ---------- Forwarded message ----------
> Date: Thu, 18 Jul 2002 15:00:43 -0700
> From: Kris Erickson <kerickso@ryerson.ca>
> To: holtsg@duke.usask.ca
> Subject: RE: Avoiding gelatine ---agar as an alternative?
>
> Hi Gord,
> Could you please post this to the list on my behalf:
>
> >Well one topic I don't think I've ever seen come up is discussion
> >on agar (also known as agar-agar). It's a gelatin-like substance
> >made from certain types of seaweed (laver, etc.--NO animal)
> >Apparently it's a fantastic size (esp. for VDB). I myself have
> >used it for single coat gums (though I have not yet perfected how
> >to apply it).
> >Personally, I'd be curious to hear about anyone's experience with
> >it, either as a sizing agent OR as an alternative to gelatine in a
> >homemade photo emulsion. Various properties,
> >similarities/dissimilarities to gelatine would all be of interest
> >to me.
> >For anyone interested, agar can be purchased in oriental grocery
> >stores (among other places), and is the main ingredient in
> >Chinese/Vietnamese jello (try the taro or mango flavours ;-) It's
> >modestly priced, but must somewhere be available inexpensively in
> >bulk. Any ideas?
>
> >kris


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