Storage of Glut. , was RE: glyoxal v. formaldehyde
Ryuji, You wrote: > Glut keeps for a long time as long as it is kept in an acidic solution > (pH of about 4 is good), in a tightly closed container, in a cool, > dark place. Not as air-sensitive as developers or formaldehyde or > glyoxal, but I'd still avoid air. ..I obtained a small unopened bottle of glutataldehyde (25%) which was kept at room temperature for an unknown period (although the label read keep below zero deg Celsius). I put it at -20degC (it freezes, a bit inconvienent), and tested it by putting 0.3 gr gelatine (bloom 300) in 10ml water, after soluting at 50degC I added 10µl 25%glut (obviously in a fume hood with proper protection). After 20 min at room temperature it became solid and I assume crosslinked. The reason for this small experiment is that I came across this paper: http://www.springerlink.com/content/l8k532p213708442/fulltext.pdf were they state that storage temperature is the main factor on the stability of glut. Do you think this suff is still good enough for sizing (guess I just have to try a small sample), or do I worry to much..;-).. Best & thanks Cor
|