From: hthomp@lsumc.edu (Thompson, Harriet)
Date: Thu, 16 May 1996 08:28:11 -0500
Subject: Re: a propos of gelatine
> This sounds similar to my problem of trying to get gelatin to stick to
> plastic sheets.
---
> I'm thinking that a very dilute solution of gelatin coating the glass
> might not form a coherent enough film to shrink and pull away from the
> glass, but would give a subsequent coat something to hold onto. This
> is pure speculation, but could be an interesting experiment.
I have lurked on this list for quite a while now, enjoying the reparte
and absorbing much of the technical information for when I can return to
my darkroom. Up until now, I haven't had anything to add to the
discussions....
In my experience in microbiology, it is common to use a very dilute
solution of agarose (a derivative of agar extracted from seaweed, and
similar in many ways to gelatin) to coat glass microscope slides prior
to the addition of a thicker, more concentrated agarose layer. The
primary coat (0.5%) is allowed to dry before pouring on the 2% layer.
Without this primary coat, the 2% layer would easily separate from the
glass slide during the several staining and washing steps. Its not
fool-proof, but superior to adherence without the 0.5% layer. BTW, for
greater success in the attachment of the layers, make sure the glass is
completely free of any oils/grease.
Harriet Thompson
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
Harriet C.W. Thompson, M.S. Louisiana State Univ. Medical Center
hthomp@lsumc.edu Microbiology/Immunology/Parasitology
New Orleans, LA http://www.lsumc.edu/campus/micr/
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